Tissue
Preparation, staining and imaging of sections of brain and spinal cord to identify which cells are present and which are active.
- Fluorescence microscopy: c-Fos immunofluorescenceFluorescence microscopy of c-Fos, NeuN, DAPI and a Nissl stain in separate colour channels, in mouse or rat brain, mouse spinal cord or human post-mortem brain.Guided experiment
- Counting neurons without bias: design-based stereologyThe experiment tests whether a group of brains has fewer neurons than a control group, for example fewer dopaminergic (TH-positive) neurons in the substantia nigra pars compacta of a mouse model of Parkinson's disease, by the optical fractionator with the animal as the unit of analysis.Guided experiment
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