RNA
Methods that measure which genes are expressed, where and in what amount, from RNA extraction to single-cell sequencing.
- RNA in situ hybridisationSingle-molecule fluorescent in situ hybridisation to identify which neurons in a region contain the mRNA of an excitatory marker, which contain the mRNA of the inhibitory marker <i>Gad1</i>, and whether the two overlap.Guided experiment
- Measuring gene expression by RT-qPCRThe messenger RNA of one gene is measured in the hippocampus of two groups, from frozen tissue to a statistical comparison: RNA extraction and quality control, reverse transcription, primer validation, the qPCR run, the threshold and melt curve, the choice of reference genes and the analysis.Guided experiment
- Finding cell types with single-cell RNA sequencingA single-cell RNA sequencing experiment is run on the cortex of control and treated mice, from the design and tissue dissociation to the naming of cell types and a test of whether the treatment changes the share and the gene expression of microglia.Guided experiment
- Single-cell RNA sequencingSingle cells from mouse cortex, analysed from quality control to cell types, with conditions compared using animals as replicates.Simulator
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