Cells and circuits
Recording, imaging and control of single neurons and small populations: patch clamp, optogenetics and calcium imaging.
- Two-photon calcium imaging of visual cortexTwo-photon calcium imaging of the responses of neurons in layer 2/3 of mouse primary visual cortex to gratings of different orientation, from the choice of indicator to the statistics.Guided experiment
- Primary neuronal culture, immunocytochemistry and Sholl analysisA test of whether a compound changes dendritic branching in cultured neurons, from the coated coverslip to a Sholl analysis with the independent culture as the unit of analysis.Guided experiment
- Controlling a neuron with light in a brain sliceThe experiment measures how a light-gated protein changes the firing of neurons in an acute brain slice: the opsin is matched to the question, expression is checked, photocurrents and light-driven spikes are recorded, the controls are run and the result is analysed with the animal as the unit.Guided experiment
- Recording a neuron with patch clampA whole-cell patch-clamp recording is made step by step, as on a real rig: the pipette is prepared, a cell is found and sealed, the quality of the recording is checked, and the firing and ionic currents of the cell are measured and tested with two classic channel blockers.Guided experiment
- Patch-clamp electrophysiologyThe voltage and ion currents of a single simulated neuron, and the effects of drugs that block its channels.Simulator
- OptogeneticsExcitation and silencing of neurons with light-gated proteins, and the limits of each tool.Simulator
- Two-photon calcium imagingImaging of a population of neurons in visual cortex and of the response of each neuron to oriented gratings.Simulator
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